Objective:To investigate the effect on skin-wound healing and explore the healing mechanism of bioactive glass...
Cai Lin 1,2,Cong Mao 1,3,Xiaofeng Chen 1,2 1.The National Engineering Research Center for Tissues Restoration and Reconstruction,Guangzhou,P.R.China,510640 2.The School of Materials Science and Engineering,South China University of Technology,Guangzhou,P.R.China,510640 3.The School of Bioscience and Bioengineering,South China University of Technology,Guangzhou,P.R.China,510640
In this research, polypyrrole nanocone arrays doped with β-Naphthalene sulphonic acid （PPy-NSA） were built. This film was expected to control protein adsorption and bacterial adhesion by potential-induced reversibly redox. The scanning Kelvin probe microscopy （SKPM） and surface contact angles （SCA） tests suggested that the surface potential and wettability of PPy-NSA nanocone arrays could be controlled by simply controlling its redox property via applying potential. The controllable surface potential and wettability in return controlled the adsorption of protein and adhesion of bacteria. The proposed material might find application in the preparation of smart biomaterial surfaces that can regulate proteins and bacterial adhesion by a simple potential switching.
Zhengnan ZhouWeiping LiTianrui HePeng YuGuoxin TanChengyun Ning
Chitosan oligosaccharides(COS)is derived from the chemical or enzymatic decomposition of chitosan.The exact mechanisms underlying many biological functions of COS have not been elucidated.Since subcellular localization is very important in determining biological activity in a number of molecules,we used fluorescein isothiocyanate-labeled chitosan oligosaccharides(FITCCOS)and investigated the localization of COS in living cells by laser scanning microscopy.We found that COS entered cells in a dose-and time-dependent manner,and we first showed that COS may enter cells by facilitated passive diffusion and was preferentially localized in the mitochondria.While in high concentration,it was also found in the cytoplasm and nucleus and was enriched in the nucleolus and karyotheca.The different subcellular localization of COS suggested that they played different effects.Determination of COS subcellular localization in cells is important to help us understand the potent mechanisms underlying its multiple functions.
