[Objective] The paper was to explore the cause of disease with symptoms of swelling joint, blanc-loose stool and few deaths, so as to provide suggestions and effective measures for diagnosis and control of the disease in chicken farm. [Method] The diseased chick samples were collected for bacteriological examination. Through bacterial isolation culture, PCR identification, biochemical test and animal test, 5 strains of S. pullorum were identified. [Result] When the isolates were inoculated into SPF chicks, the same disease cases with natural infection could be reproduced. [Conclusion] S. pullorum is the major cause of the disease in chicken farm.
为了探索不同血清型副鸡禽杆菌菌株外膜蛋白的差异,本试验采用FOCUSTM Global Fractionation试剂盒制备副鸡禽杆菌A(221株)、B(0222株)、C(Modesto株)3个血清型参考菌株的外膜蛋白,进行SDS-PAGE凝胶电泳。结果表明,不同血清型参考菌株的电泳图谱相似,经凝胶定量软件分析,结果显示,主要条带均有7个,其分子质量相似,分别占A、B和C蛋白质总量的70.85%、68.6%和65.71%。此外共有3个蛋白质条带分子质量相似,但蛋白质含量差异显著(P<0.05)。本试验为副鸡禽杆菌外膜蛋白的进一步研究提供了理论依据。
