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国家自然科学基金(30901342)

作品数:3 被引量:13H指数:1
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利妥昔单抗治疗系统性红斑狼疮出现血栓栓塞并文献复习
2013年
目的与利妥昔单抗相关血栓的不良反应非常罕见,通过学习1例使用利妥昔单抗后出现严重血栓事件患者的临床特点,进一步了解利妥昔单抗的不良反应。方法详细报道1例系统性红斑狼疮(systemic lupus erythematosus,SLE)合并抗磷脂抗体综合征(anti phospholipid syndrome,APS)患者使用利妥昔单抗后出现血栓的临床表现。结果该患者在使用利妥昔单抗治疗3个月后,PLT正常,狼疮不活动,但出现下肢深静脉血栓和肺栓塞。结论利妥昔单抗治疗SLE有效,但给予SLE合并APS患者使用时要警惕血栓形成的风险。
苏金梅陈华徐东周炜洵张烜张奉春
关键词:利妥昔单抗血栓狼疮
PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma被引量:13
2012年
Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tis- sues and to validate its bona fide targets in human cholangiocarcinoma cells. Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholan- giocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respec- tively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarci- noma tissues by using real-time PCR, locked nucleic acid in situ hybridization (LNA-ISH), and immunohis- tochemistry analysis. Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bileduct tissues (P〈0.05). The dual luciferase reporter gene assay revealed endogenous microRNA-21 in cholan- giocarcinoma cell line, QBC939, inhibited the luciferase reporter activities of wild-type PTEN (P〈0.01) and PDCD4 (P〈0.05) and had no this effect on mutated PTEN and PDCD4. Moreover, loss of microRNA-21 function led to a significant increase of PTEN and PDCD4 protein levels in QBC939 cells. Elevated microRNA-21 levels were accompanied by marked reductions of PTEN and PDCD4 expression in the same cholangiocarcinoma tissue. Conclusion microRNA-21 expression is up PDCD4 are direct effectors of microRNA-21. regulated in human cholangiocarcinoma and PTEN,
Chang-zheng LiuWei LiuYi ZhengJin-mei SuJing--jing LiLan YuXiao-dong HeSong-sen Chen
关键词:CHOLANGIOCARCINOMAMICRORNA-21
Expression of microRNA-29b2-c Cluster is Positively Regulated by MyoD in L6 Cells
2013年
Objectives To evaluate the expression profile of myoD microRNA-29 (miR-29) family in L6 myoblast differentiated to myotube or L6 myotube treated by glucose and insulin, and to further probe the molecular mechanism of myoD regulating the expression of miR-29 clusters.
Chang-zheng LiuJing-jing LiJin-mei SuTao JiaoLi-juan GouXiao-dong HeYong-sheng Chang
关键词:MYOGENESISMYOD
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