This study was performed to investigate the effects of the cyclonic separation mechanism on the removal of magnesium from phosphate ore. An analysis of the particle-bubble collision mechanism in a cyclonic force field was used to design two different pulp circulation systems for a cyclonic-static micro-bubble flotation column (FCSMC). Experiments comparing the two conditions were then conducted. Size analysis of the feed and separated products was conducted with a BXF cyclone size analyzer. The results show that about 34.60% of the total MgO content in the feed exists in the -10 μm fraction. This demonstrates that magnesium removal from phosphate ore has the characteristics of a micro-fine particle flotation problem. Under conditions of cyclonic circulation the MgO level of the concentrate is 1.74g, which is lower than that obtained from the column operated in direct-flow circulation by 0.34%, These results indicate that the cyclonic circulation mechanism of the FCSMC can promote removal of micro-fine particles containing magnesium. This is attributed to the function of surface flotation and to the reduction of the lower separation limit under conditions of cyclonic circulation.