General situation of Jinghong City was introduced in this study.Based on analyzing its vegetation components,species diversity,rare and endangered species,alien species,plant resources,7 biodiversity conservation contents were planned,including focusing on the construction and management of nature reserves,constructing forest park and developing science popularization engineering,establishing the area of banning hunting(picking) wild species and connection corridors,constructing wild elephant park into a zoo,promoting the role of urban parks in protecting biodiversity,devoting more in protecting rare and endangered species,preventing the danger of invasive species.4 countermeasures for applying biodiversity in urban green spaces were proposed,specifically,creating urban garden plant community by simulating natural ones to form urban plant ecosystem,determining species cardinal of urban garden plants to protect and develop species diversity,applying both local and alien plants to create diversified landscapes,introducing,domesticating or culturing new garden plants to enrich the diversity of garden plants.Also 3 countermeasures for protecting biodiversity in urban green spaces were put forward,that is,joint development of rural economy and biodiversity protection around cities,protecting species and comprehensively renovating habitats,fully utilizing ex-situ conservation to protect biodiversity.