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国家自然科学基金(30570188)

作品数:3 被引量:8H指数:1
相关作者:田桂红仲英周玲徐万崔淑香更多>>
相关机构:山东省医学科学院济南大学山东医学高等专科学校更多>>
发文基金:国家自然科学基金更多>>
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霞草苷对人结肠癌HT-29细胞生长和凋亡的影响被引量:1
2014年
目的观察霞草苷对人结肠癌HT-29细胞凋亡的影响及其可能机制。方法用MTT法和流式细胞技术检测浓度为5,10,20,40μmol·L-1霞草苷对HT-29细胞生长、凋亡的影响;用实时荧光定量RT-PCR检测细胞Caspase-3、Bax的mRNA水平。结果霞草苷对HT-29细胞的生长有明显的增殖抑制作用,呈浓度和时间依赖性,细胞凋亡率明显增高。Caspase-3和Bax的mRNA表达量逐渐增加,呈明显的浓度依赖性。结论霞草苷能诱导体外培养的人结肠癌HT-29细胞凋亡,上调Bax表达,活化Caspase-3可能是霞草苷诱导HT-29细胞凋亡的机制之一。
田桂红秦迎周玲仲英牟艳玲
关键词:结肠癌细胞凋亡半胱氨酸蛋白酶-3
Experimental Studies of the Therapeutic Effect of Gypsophila Oldhamiana Gypsogenin on Lewis Lung Cancer in Mice被引量:1
2008年
OBJECTIVE To observe the inhibitory effect of Gypsophila oldhamiana gypsogenin (GOG) on Lewis lung cancer growth,and to investigate the mechanism of its anti-tumor effect. METHODS A mouse model bearing Lewis lung cancer was used.The 5 experimental groups were divided into a positive control (cis-diaminedichloroplatinum),a negative control,and high,medium and low-GOG dosage groups.The inhibitory action of GOG administration on the lung cancer was observed.After GOG treatment,the lungs were taken out and a lung coefficient computed.The expressions of VEGF,Bcl-2,Bax and P53 in the cancers were determined using immunohistochemical staining. RESULTS The tumor weight of the mice given various doses of GOG was significantly lower compared to the negative-control group (P<0.01),and the lung coefficient of the groups given high and low GOG doses was significantly lower compared to the negative-control group (P<0.01).Immunohistochemical results were shown as follows:i) The VEGF and Bcl-2 expressions in the GOG groups were significantly lower than that of the negative-control group (P<0.05);ii) Bax expression in the groups treated with high and medium GOG doses was significantly higher compared to the negative-control group (P<0.05);iii) The mutant P53 expression in the GOG-treated groups was significantly lower compared to the negative-control group (P<0.05).CONCLUSION GOG can inhibit the growth and metastasis of Lewis lung cancer,and may exert its mechanism of tumor control by inhibition of tumor angiogenesis and induction of apoptosis.
Guihong Tian Ling Zhou Ying Zhong Wan Xu Hong Bai Lu Liu Shuxiang Cui
霞草皂苷对A549细胞和小鼠Lewis肺癌的抑制作用被引量:6
2013年
利用MTT法和流式细胞技术分析霞草皂苷对A549肺癌细胞生长及凋亡的影响;建立小鼠Lewis肺癌模型,观察霞草皂苷对Lewis肺癌的抑制作用,探讨其抗癌机制。结果表明:霞草皂苷对A549细胞株有显著的生长抑制作用,呈一定的量效依赖性,IC50为0.19 mg/L;霞草皂苷3个剂量组干预的小鼠肿瘤的质量及2个剂量组的肺系数明显低于阴性对照组(p<0.01),免疫组化结果显示霞草皂苷各剂量组血管内皮生长因子(VEGF)和突变型P53基因表达明显低于阴性对照组(p<0.05)。霞草皂苷抑制肺癌生长机制可能和VEGF及P53基因有关。
田桂红周玲周玲仲英崔淑香
关键词:A细胞LEWIS肺癌肿瘤抑制
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