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作品数:9 被引量:49H指数:4
相关作者:杨仕明韩东一翟所强杨伟炎陈伟更多>>
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发文基金:国家自然科学基金国家高技术研究发展计划“十一五”国家科技支撑计划更多>>
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Effects of combined administration of furosemide and kanamycin on rat auditory nerve被引量:1
2011年
Objective To determine the effects of combined administration of furosemide and kanamycin on inner ear structures and the auditory nerve in rats. Methods The rats in the treatment group received intravenous injections of combined furosemide and kanamycin sulfate, and the rats in the normal control group received no treatment. The auditory brainstem response (ABR) test was carried out 7 days after drug administration to determine the effects of drug administration on hearing. Cochlear slice and cochlear wholcmount were prepared after 7 days of drug treatment. Results After 7 days of drug administration, ABR thresholds were significantly higher in the treatment group than in the control group and neurofilaments were significantly reduced, although the number of spiral ganglia showed no decrease and there were no signs of supporting cell injury. Conclusions Combined administration of furosemide and kanamyein sulfate has an apparent synergistic ototoxic effect. Although spiral ganglion damage may not be apparent within a short time period of drug administration, damage to auditory nerve fibers is obvious.
GUO WeiweiYUAN Fen-qianLIU Hui-zhanYANG Shi-ming
关键词:KANAMYCINFUROSEMIDE
老年及老年前期人工耳蜗植入的临床初步研究被引量:20
2010年
目的 探索人工耳蜗在双耳重度或极重度聋和全聋的老年患者中的临床应用安全性和效果.方法 2008年11月至2009年11月解放军总医院先后对8例50岁以上患者行人工耳蜗植入术,其中男5例,女3例;年龄52~76岁,中位数58岁.所有患者术前均为双侧极重度感音神经性听力损失,佩戴助听器无效或效果不佳.观察患者手术耐受及并发症情况.开机后3个月进行助听听阈评估和言语评估.结果 8例患者手术顺利,术中神经反应遥测均引出反应.术后康复顺利,无任何并发症.开机3个月后声场内言语频率平均助听听阈啭音(听力级)为35~50 dB,但言语测听结果个体差异较大.部分患者单音节、双音节、安静及噪声环境下的句子可实现较好识别率.结论 老年前期及老年患者可耐受全身麻醉状态下的人工耳蜗植入,只要术前准备充分则为安全的手术,但部分病例为中耳炎(胆脂瘤)或颞骨外伤等因素导致,需要特别注意中耳情况.老年重度以上听力损失患者人工耳蜗植入可明显改善言语识别能力.
杨仕明李佳楠冀飞陈艾婷洪梦迪戴朴郗昕韩东一翟所强
关键词:耳蜗植入术老年人听觉丧失耳外科手术
An investigation into hearing loss among patients of 50 years or older
2011年
Objects To investigate the extent of hearing loss in an elderly sample population to estimate hearing disorders among the age-equivalent population in China and to study primary clinical characteristics of presbycusis. Methods Clinical data from 110 hearing loss patients of both senium and pre-senium ages (95 males and 15 females, mean age = 74.4±12.1 years) were reviewed. Patients aged from 50 to 59 years were accepted as the pre-seni- um control group (n=15). The 95 senium patients (〉 60 years of age) were divided into 4 groups according to age: 60+ group(60 to 69 years, n=25), 70+ group (70 to79 years, n=26), 80+ group (80 to 89 years, n=32) and 90+ group (90 years or older, n=12). Pure tone audiometry thresholds were measured in all 110 patients. Hearing loss severity of each tested ear was rated according to the Goodman classification criteria. Besides, audiometric configuration was examined in each ear. Results Audiometric testing showed normal hearing in 65 ears (29.5%), slight to moderately severe hearing loss in 131 ears (59.5%), and severe and profound loss in 24 ears (11%). Air-bone gaps were found in 12 ears (5.45%) indicating conductive hearing loss. Except the 12 ears with conductive hearing loss, audiograms showed gradually sloping loss in 99 ears (45%), sharply sloping loss in 34 ears (15.45%), fiat loss in 45 ears (20.45%), notch pattern loss in 5 ears(2.27%), trough and rising pattern loss in 2 ears(0.91%), total deafness in 2 ears (0.91%), and normal hearing in 21 ears(9.55%). On average, hearing thresholds increased at a rate of approximately 10 dB per 10 year for subjects aged 60 and older. Conclusions Hearing thresholds tend to be stable in presbycusis patients aged from 50 to 70 years, increase significantly between 70 and 80 years of age, and reach another stable stage at high levels after 80 years of age, especially in high frequencies. Hearing loss in middle frequencies accounts for most of recession in
JI Fei LEI Lei ZHAO Su-ping LIU Ke-fang ZHOU Qi-you YANG Shi-ming
关键词:ELDERLYPRESBYCUSIS
耳蜗内毛细胞突触对氨基糖甙类药物毒性的反应特性被引量:10
2010年
目的探讨氨基糖甙类药物毒性对小鼠耳蜗内毛细胞突触数量的影响。方法采用固定剂量(100mg/kg)的庆大霉素对C57BL/6J小鼠每日进行腹腔注射造模,分别在注射后的第4天、7天和10天观察耳蜗基底膜顶回区域的内毛细胞突触数量的变化,以同窝未注射小鼠的耳蜗基底膜顶回作为实验对照。内毛细胞突触前后膜分别采用CtbP2和GluR2/3进行免疫荧光标记,在激光共聚焦显微镜下进行观察。采用3dmax8.0对实验结果进行三维重建,在此基础上计数耳蜗内毛细胞突触的数量。结果所有小鼠内外毛细胞均无缺失和错排,但和对照组相比,内毛细胞突触数量存在明显变化(P<0.01);其数量在庆大霉素注射第7天时达到峰值,之后明显减少。结论在氨基糖甙类药物毒性环境中,耳蜗内毛细胞突触数量表现先增加后下降的规律。这提示在氨基糖甙类药物毒性作用的过程中,内毛细胞突触数量的增加可能表明耳蜗内毛细胞带状突触存在通过改变自身数量来反映氨基糖甙类药物毒性损害的机制。
柳柯姜学钧李姝娜吴南刘会占张悦杨伟炎杨仕明
关键词:庆大霉素耳蜗激光共聚焦显微镜
The progenitors of inner ear hair cells and their regulating genes
2010年
Hair cells in the mammalian inner ear are very fragile and are often injured as a result of acoustic trauma or exposure to ototoxic drugs (cisplatin, aminoglycosides, etc) [1]. In amphibians and birds, spontaneous
SHU Wei-ningZHAO Li-dongZHANG Xiao-bingYANG Shi-ming
关键词:REGENERATION
Optical recordings reveal the differential distribution of glutamatergic transmission in the cochlear and vestibular nuclei in the newborn mouse brainstem
2009年
Objective Using optical imaging in brainstem slices, we studied evoked responses in the mouse cochlear (CN) and vestibular (VN) nuclei. Methods The use of optical imaging allowed us to visualize the spatiotemporal patterns of excitatory propagation in the CN and VN. Optical recordings can differentiate excitatory propagation in the ventral CN(VCN) from that in the dorsal CN(DCN). Furthermore, we assessed the relative distribution of NMDA and non-NMDA receptors in these regions using the glutamate antagonists APV (NMDA receptor antagonist) and CNQX (non-NMDA receptor antagonist) in mouse brainstem slices during postnatal days 1 to 3. Results The average sensitivity to APV was 99.1% in the VCN, 76.0% in the DCN and 64.9% in the VN. The average sensitivity to CNQX was 0.9% in the VCN, 24.0% in the DCN and 35.1% in the VN. These results indicate that the effect of APV dominated in the VCN, while both APV and CNQX were effective antagonists in the DCN and VN. In the VN, the distribution of APV-and CNQX-sensitive cells was almost completely uniform. However, in the DCN the distribution of APV-and CNQX-sensitive cells was highly complex. The area that was more sensitive to CNQX was located in the superficial layer of the DCN while the area with a higher sensitivity to APV was located progressively in the deep layer. Conclusion This optical recording data suggests that there is a differential distribution of NMDA and non-NMDA receptor mediated neurotransmission in the VCN, DCN and VN.
Doi TadashiKaneko ToshihikoMatsumoto AyumiAsako MikiyaYamashita Toshio
关键词:NMDA
Math1基因内耳导入后噪声性聋豚鼠听功能改变观察被引量:17
2009年
目的观察Math1基因内耳导入对噪声性聋豚鼠听功能的影响,探讨Math1基因过表达对噪声损伤耳蜗的生物学效应,为内耳基因治疗提供实验基础和理论依据。方法经脉冲噪声致聋的豚鼠45只(各频率ABR阈值均≥95dB SPL),雌雄不限,实验开始时体重250~300g。随机分为3组:Ad-Math1-EGFP组(30只);Ad-EGFP组(5只);空白组(10只)。各组豚鼠在基因转导后4周、8周分别测试双耳ABR。测试完毕后处死动物,观察听泡及耳蜗无炎性病变者记录听阈结果。结果Math1导入后4周,导入耳各频率ABR阈值低于对照耳(右耳),也低于Ad-EGFP组及空白组,平均达到85dB SPL。Math1导入后8周,导入耳各频率ABR阈值低于对照耳(右耳),也低于Ad-EGFP组及空白组,与4周时比较,进一步好转,平均达到75dB SPL。结论Math1基因内耳导入可使噪声导致全聋的豚鼠听功能部分恢复,为噪声性聋的治疗打开了新的思路和手段。
陈伟郭维维胡吟燕孙建和于宁韩东一翟所强杨伟炎何志洲杨仕明
关键词:基因转导噪声性聋MATH1ABR
Endoscope-Assisted Cerebellopontine Angle Surgery被引量:1
2009年
Objective To report experiences with use of otoendoscopy in cerebellopontine angle(CPA) surgeries.Methods Twenty five cases of CPA surgeries performed between November 2002 and December 2008 in which microscope enabled otoendoscopy was used were reviewed.The 25 cases included 19 cases of acoustic neuroma,3 cases of CPA facial nerve tumors,1 case of trigeminal neurinoma,a case of glossopharyngeal neuralgia and 1 case of hemifacial spasm.Endoscopy was used in all cases together with monitoring of brainstem auditory responses and facial electromyography.Postoperative hearing and facial nerve function were evaluated and compared to pre-operative levels.Results Endoscopy provided improved visualization of local anatomy,revealed hidden lesions and reduced unnecessary anatomical distortions.Total resection was achieved in 18 of the 19 acoustic neuroma cases,Facial nerve anatomical integrity was preserved in all 19 cases.One week postoperative House-Brackmann grading was I in 3 cases,Ⅱ in 10 cases and Ⅲ in 6 cases.Facial nerve function continued to improve in some cases at 3 months.Total tumor resection was achieved in all 3 patients with facial neurinoma.The facial nerve was sacrificed in 2 of the 3 cases with primary faciohypoglossal nerve anastomosis.Facial nerve function was Grade Ⅱ and Grade III one year after surgery,respectively.In the case with anatomically preserved facial nerve,postoperative facial nerve function was initially Grade Ⅲ and improved to Ⅱ at 3 months.The tumor was completely resected in the trigeminal neurinoma patient with a Grade Ⅲ postoperative facial nerve function which improved Grade II three months later.Seventeen of the 19 patients with acoustic neuroma retained hearing postoperatively,of these 12 maintained preoperative levels of hearing.Preoperative hearing capacity was preserved in 2 of the 3 patients with facial nerve tumors,but lost in patients with other tumor types.Glossopharyngeal neurotomy(n=1) and microvascular decompression(n=1) resulted in satisfactory symptom relie
YANG Shi-ming,YU Li-Mei,ZOU Yi-hui,YU Li-Ming,JI Fei,YANG Wei-yan,HAN Dong-yi Dept.Otolaryngology Head and Neck Surgery,Institute of Otolaryngology,Chinese PLA General Hospital,Beijing 100853
关键词:CPAENDOSCOPE
Math1基因内耳导入径路的探索研究被引量:5
2009年
目的研究腺病毒携带Math1-EGFP基因经完整圆窗膜途径及鼓阶打孔途径导入耳蜗后对听功能和转导效率的影响,为内耳基因治疗提供实验基础和理论依据。方法健康成年白色红目豚鼠40只,雌雄不限,体重250~300g。随机分成四组,完整圆窗膜组12只,鼓阶打孔组12只,各组分别设对照8只。实验组(24只)导入重组腺病毒携带的Math1基因及增强型绿色荧光蛋白基因(enhanced green fluorescent protein,EGFP),对照组(16只)导入人工外淋巴液,所有动物均以左耳作为导入耳。术前及术后分别行听性脑干反应(ABR)检查。分别于术后5天、14天取双侧耳蜗标本做基底膜铺片观察基因表达情况。结果完整圆窗膜组导入耳ABR阈值,术后5天各频率与术前比较无显著性差异(P>0.05);鼓阶打孔组导入耳ABR阈值,术后5天在2kHz、4kHz与术前比较无差异(P>0.05),8kHz较术前增高(P<0.05),16kHz、20kHz较术前明显增高(P<0.01),术后14天在16kHz、20kHz较术后5天时明显好转(P<0.01),但较术前仍有增高(P<0.05)。转导成功率鼓阶打孔组为91.6%,优于完整圆窗膜组的50%。两种转导途径对目的基因在耳蜗内的表达部位和表达时间没有显著影响。结论完整圆窗膜途径及鼓阶打孔途径在转导成功率及听功能保护方面各有优劣。完整圆窗膜途径因其对耳蜗的损伤极小,在临床应用方面具有更好的发展前景。
陈伟郭维维郭维维胡吟燕孙建和于宁李兴启韩东一翟所强韩东一
关键词:基因转导耳蜗腺病毒
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