目的:研究钾离子通道阻滞剂四乙胺(T EA )在体外是否具有抗胶质瘤作用,对胶质瘤细胞是否具有抑制肿瘤细胞增殖的作用。方法四甲基噻唑蓝比色法(MTT 法)观察 TEA对胶质瘤细胞生长、增殖的影响。结果 MTT法发现 TEA可显著抑制胶质瘤细胞株的生长,且呈时间和剂量依赖性。结论 T EA在体外可显著抑制C6与9L胶质瘤细胞株增殖,其作用机制可能和细胞凋亡有关。
Most axons in the vertebral central nervous system are myelinated by oligodendrocytes.Myelin protects and insulates neuronal processes,enabling the fast,saltatory conduction unique to myelinated axons.Myelin disruption resulting from trauma and biochemical reaction is a common pathological event in spinal cord injury and chronic neurodegenerative diseases.Myelin damage-induced axonal conduction block is considered to be a significant contributor to the devastating neurological deficits resulting from trauma and illness.Potassium channels are believed to play an important role in axonal conduction failure in spinal cord injury and multiple sclerosis.Myelin damage has been shown to unmask potassium channels,creating aberrant potassium currents that inhibit conduction.Potassium channel blockade reduces this ionic leakage and improves conduction.The present review was mainly focused on the development of this technique of restoring axonal conduction and neurological function of demyelinated axons.The drug 4-aminopyridine has recently shown clinical success in treating multiple sclerosis symptoms.Further translational research has also identified several novel potassium channel blockers that may prove effective in restoring axonal conduction.