鱼类精子超低温冷冻保存对种质资源保护意义重大,但面临诸多技术挑战。本研究旨在探索优化鱼类精子超低温冷冻保存技术,以提高保存效果。明确了超低温冷冻保存原理,分析了影响因素,包括抗冻剂和稀释液的选择、冷却与解冻速率等。对比了新型抗冻蛋白样液与传统样液的优劣。研究结论表明,鱼类精子超低温冷冻保存技术为鱼类种质资源保护提供了重要手段,但仍需不断优化。未来可从保存液配方、冷冻和解冻技术、细胞损伤机制及新技术应用等方面深入研究。Ultra-low temperature cryopreservation of fish sperm is of great significance for germplasm resources protection, but it also faces many technical challenges. The purpose of this research is to explore and optimize the ultra-low temperature cryopreservation technology of fish sperm so as to improve the preservation effect. The principle of ultra-low temperature cryopreservation was clarified, and the influencing factors were analyzed, including the selection of antifreeze agents and diluents, the cooling and thawing rates, etc. The advantages and disadvantages of the new antifreeze—protein-like solution and the traditional like solution were compared. The research conclusion shows that the ultra-low temperature cryopreservation technology of fish sperm provides an important means for fish germplasm resources protection, but it still needs to be continuously optimized. In the future, in-depth research can be carried out from the aspects of preservation solution formulation, freezing and thawing technologies, cell-damage mechanisms and the application of new technologies.
