Objective: to explore whether active nursing measures during perioperative period can accelerate the recovery of patients with ameloblastoma of the mandible after vascularized fibular flap transplantation. Methods: sixty-seven patients with mandibular ameloblastoma from January 2015 to May 2019 were selected and randomly divided into the intervention group and the control group. The control group received routine perioperative nursing measures after fibular flap surgery, while the intervention group received auxiliary preoperative and postoperative nursing intervention on the basis of general perioperative routine nursing measures, and received joint and muscle strength function training in the donor bone area, and the explanation of the steps and precautions of rehabilitation function training. The Prism Graphpad 7.01 software was used to analyze the postoperative pain score, the change of postoperative negative pressure drainage flow, the time of indwelling drainage tube at the flap removal and the postoperative hospital stay of the two groups of patients. Results: compared with the control group, the pain degree of the intervention group decreased significantly on the second and third days after surgery, and the negative pressure drainage volume decreased significantly. Compared with the control group, the mean postoperative duration of negative pressure drainage tube in the intervention group was shortened by 3 days, and the mean postoperative hospital stay was decreased by 5.5 days. Conclusion: nursing intervention in patients with ameloblastoma of the mandible during perioperative period can accelerate the recovery of patients.